更新時間：2025-06-06
UbiqBio熒光探針泛素Ub-Rh110MP：是脫泛素酶（DUBs）的淬滅熒光底物。泛素的Gly76和羅丹明110部分之間的酰胺鍵的斷裂釋放出高度熒光的Rh110嗎啉羰基，其熒光強度高于Ub-Rh110Gly的經典Rh110Gly熒光團（UbiQ-002）。
中文介紹：
UbiqBio熒光探針泛素Ub-Rh110MP：Ub-Rh110MP（UbiQ-126）是脫泛素酶（DUBs）的淬滅熒光底物。泛素的Gly76和羅丹明110部分之間的酰胺鍵的斷裂釋放出高度熒光的Rh110嗎啉羰基，其熒光強度高于Ub-Rh110Gly的經典Rh110Gly熒光團（UbiQ-002）。UbiQ-126是通過全化學合成制備的。
英文介紹：
UbiqBio熒光探針泛素Ub-Rh110MP：Ub-Rh110MP (UbiQ-126) is a quenched, fluorescent substrate for deubiquitylases (DUBs). Cleavage of the amide bond between Gly76 of ubiquitin and the Rhodamine110 moiety releases the highly fluorescent Rh110-morpholinecarbonyl which exhibits a higher fluorescence intensity than the classical Rh110Gly fluorophore of Ub-Rh110Gly (UbiQ-002). UbiQ-126 is prepared by total chemical synthesis.
產品被引文獻：
1.Hassiepin, U., et al. A sensitive fluorescence intensity assay for deubiquitinating proteases using ubiquitin-rhodamine 110-glycine as substrate. Anal. Biochem. 371, 201-207 (2007). ncbi.nlm.nih.gov/pubmed/17869210
2.El Oualid, F., et al. Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin. Angewandte Chemie Int. Ed. 49, 10149-10153 (2010). ncbi.nlm.nih.gov/pubmed/21117055
3.Lavis, L.D., et al. Fluorogenic label for biomolecular imaging. ACS Chem. Biol. 1, 252-260 (2006). ncbi.nlm.nih.gov/pubmed/17163679
4.Terentyeva, T.G., et al. Morpholinecarbonyl-Rhodamine 110 based substrates for the determination of protease activity with accurate kinetic parameters. Bioconj. Chem. 22, 1932-1938 (2011). ncbi.nlm.nih.gov/pubmed/21905728
